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tgl [2018-05-03 08:40:30]
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tgl [2018-05-03 08:40:30]

outer spore coat protein, spore associated transglutaminase
locus
BSU31270
pI
6.69
mw
28.15 kDa
protein length
245 aa Sequence Blast
gene length
735 bp Sequence Blast
function
introduction of crosslinks in the spore coat protein GerQ
product
spore associated transglutaminase
essential
no
ec
2.3.2.13
synonyms
yuxF,yugV
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.5|Class V]

    • Gene

    Coordinates
    3,212,591 → 3,213,328

    The protein

    Catalyzed reaction/ biological activity

  • cross-links [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|SafA] to other spore coat proteins [pubmed|29712873]
  • Protein glutamine alkylamine = protein N(5)-alkylglutamine NH3 (according to Swiss-Prot), temperature-dependent modification of the coat proteins such as [protein|1CA990DA55B6F879968C431A703E0478F5564A1A|GerQ] (together with [protein|A46D07F8F56249A04C5F880120D19F00C9CAE112|YabG]) [Pubmed|16751597]

    • Protein family

  • bacillus TGase family (according to Swiss-Prot)

    • Structure

  • [PDB|4P8I] [Pubmed|26322858]
  • [PDB|4PA5] (complexed with cystamine) [Pubmed|26322858]

    • [SW|Localization]

  • outer spore coat, localization depends on [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|CotE] [Pubmed|22171814]
  • inner spore coat and cortex, dependent on [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|SafA] [pubmed|29712873]

    • Expression and Regulation

    Operons

    genes
    [gene|4A2B1DA38608E5A4096087BF7246D4C64C54DBE3|tgl]
    description
    [Pubmed|9274030]

    sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,12501297], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15383836], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: repression, [Pubmed|15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

    • regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|15383836,15699190,12501297]
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    Biological materials

    Mutant

  • MGNA-A615 (tgl::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/615 NBRP B. subtilis, Japan]
  • 1S122 ( ''tgl''::''erm''), [Pubmed|15317760], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S122&Search=1S122 BGSC]
  • BKE31270 (Δ[gene|4A2B1DA38608E5A4096087BF7246D4C64C54DBE3|tgl]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTAGCCCCCCTTGTT, downstream forward: _UP4_ATCGTCCGCTAAAAAGCCCC
  • BKK31270 (Δ[gene|4A2B1DA38608E5A4096087BF7246D4C64C54DBE3|tgl]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTAGCCCCCCTTGTT, downstream forward: _UP4_ATCGTCCGCTAAAAAGCCCC

    • Labs working on this gene/protein

  • [SW|Adriano Henriques], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]

    • References

    Reviews

  • 19797877

    • Original Publications

  • 18249137,22171814,12501297,16267299,11193401,16751597,9274030,15699190,15383836,26322858,29712873